Primer

Part:BBa_K391003

Designed by: Phillip Chu   Group: iGEM10_British_Columbia   (2010-10-25)

Reverse Primer to PCR agrCA (with RBS) from S. aureus

This is the reverse primer to PCR agrCA. Together with the forward primer (BBa_K391002), it is possible to PCR agrCA from S. aureus strain NCTC 8325. Add SpeI and PstI before using. UBC iGEM was able to obtain correct bp PCR product, but was not able to clone the product to a BioBrick vector.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None