Primer

Part:BBa_K391002

Designed by: Phillip Chu   Group: iGEM10_British_Columbia   (2010-10-25)

Forward Primer to PCR agrCA (with RBS) from S. aureus

This is the forward primer for agrCA PCR. Add EcoRI and XbaI sites before using. Along with the reverse agrCA primer (K391003), it is possible to obtain agrCA from S. aureus. Correct bands were obtained using these primers by UBC iGEM, but the cloning to a BioBrick vector failed. The orientation of agrCA is agrC first.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None