Primer
Part:BBa_K391002
Designed by: Phillip Chu Group: iGEM10_British_Columbia (2010-10-25)
Forward Primer to PCR agrCA (with RBS) from S. aureus
This is the forward primer for agrCA PCR. Add EcoRI and XbaI sites before using. Along with the reverse agrCA primer (K391003), it is possible to obtain agrCA from S. aureus. Correct bands were obtained using these primers by UBC iGEM, but the cloning to a BioBrick vector failed. The orientation of agrCA is agrC first.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
[edit]
Categories
Parameters
None |