Intermediate

Part:BBa_K390062

Designed by: Cody Tramp   Group: iGEM10_Utah_State   (2010-08-01)


GFP with art+ secretion tag for Synechocystis sp. PCC 6803

Cycle 3 mutant GFP with artifically designed secretion tag (Sergeyenko TV and Los DA. 2003. Cyanobacterial leader peptides for protein secretion. FEMS Microbiology Letters 218: 351-357) for use in Synechocystis sp. PCC 6803.

This should be a highly secreted construct (verification pending).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 74
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 593
    Illegal XhoI site found at 494
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None