Intermediate

Part:BBa_K390028

Designed by: Cody Tramp   Group: iGEM10_Utah_State   (2010-08-01)

GFP with 5'UTR and native RBS from Synechocystis sp. PCC 6803

This part is the cycle 3 mutant GFP (BBa_K208000) with the Synechocystis 5'UTR and native RBS (BBa_K390001).

It will be preceded by a series of promoters to assess their expression levels and conditions of activation.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 59
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 578
    Illegal XhoI site found at 479
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None