Regulatory

Part:BBa_K390006

Designed by: Cody Tramp   Group: iGEM10_Utah_State   (2010-08-01)

sigA Type I promoter from Synechocystis sp. PCC 6803

This is the promoter for the sigma factor sigA in Synechocystis sp. PCC 6803. It is self-regulating (controlled by SigA), but has shown to potentially be down-regulated due to changes from heat-shock, darkness, and salt stress. A decrease in SigA protein was observed in a SigC knockout, but not in direct proportion to the amount of SigA transcript (Imamura S and Asayama M. 2009. Sigma Factors for Cyanobacterial Transcription. Gene Regulation and Systems Biology 3:65-87).

As SigA is the housekeeping sigma factor for Synechocystis sp. PCC 6803, this promoter is likely constitutive, with a moderate level of expression (this is pending experimental verification).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None