Tag

Part:BBa_K390004

Designed by: Cody Tramp   Group: iGEM10_Utah_State   (2010-08-01)


art+ secretion tag for Synechocystis sp. PCC 6803

In Synechocystis sp. PCC 6803, the system of secretion is based on the charge of the N-terminus leader sequence. Positive charges on the N-terminus leader sequence contribute to secretion in a non-sequence-dependent manner (Sergeyenko TV and Los DA. 2003. Cyanobacterial leader peptides for protein secretion. FEMS Microbiology Letters 218: 351-357).

This part is an artificially designed secretion tag from the above paper. It is composed of positively charged amino acids, and was shown to successfully function as a secretion tag in Synechocystis in the above paper.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
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