Composite

Part:BBa_K389413

Designed by: Jonas Aretz   Group: iGEM10_Bielefeld-Germany   (2010-10-11)

vir promoter + sensitivity tuner + luciferase

This part combines a mutated virG response regulator from the VirA/G receptor system under the control of a constitutive promoter with a sensitivity tuner before a luciferase gene under the control of a vir promoter. The sensitivity tuner enhances a signal from a promoter so it enhances the readout luciferase.

By modifying the Sensitivity Tuner the RBS from the phage activator from the original Sensitivity Tuner was removed. New RBS comes with Virb-promotor element K389003.


Usage and Biology

For a working VirA/G-receptor system, you also need the virA BioBrick BBa_K389001. You can find the complete working system here: BBa_K389423


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 2455
    Illegal NgoMIV site found at 3799
    Illegal NgoMIV site found at 3820
    Illegal AgeI site found at 1978
    Illegal AgeI site found at 2090
    Illegal AgeI site found at 2292
    Illegal AgeI site found at 3523
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 3705


[edit]
Categories
//cds/transcriptionalregulator/activator
//chassis/prokaryote/ecoli
//function/reporter/light
//regulation/positive
Parameters
None