Composite

Part:BBa_K3893027

Designed by: Jean Pierre Herdoiza   Group: iGEM21_Ecuador   (2021-10-16)


AHL induced expression of Lysis protein.

domestication.
Figure 1. SBOL Visual representation of the part


AHL induced transcriptional unit assembled with a one-pot Level 1 Golden Gate reaction using BsaI type IIS endonuclease.

This composite part, together with K3893026, is a component of the intermediate part K3893029 to assemble the release system device (K3893030) of our Agrobactory 593. It is used to express the lysis protein phiX174 when AHL (bound to to LuxR) is present, a fundamental piece of our population oscillator. release device.

This transcriptional unit is composed of the following standardized parts from Valencia_UPV 2018 iGEM Team Part Collection:

  • BBa_K2656003: the R0062 inducible pLux promoter in its Golden Braid compatible version
  • BBa_K2656010: the B0032 weak ribosome biding site in its Golden Braid compatible version
  • BBa_K2656015: the phiX174 lysis protein
  • Terminator BBa_K2656026: the B0015 transcriptional terminator in its Golden Braid compatible version

Part BBa_K2656015 is the lysis gene from the enterobacteria phage phiX174 coding sequence. The lysis gene E of coliphage PhiX174 encodes a 91-amino acid (aa) residue protein of approximately 10 kDa that mediates inhibition of peptidoglycan biosynthesis of Gram-negative bacteria membrane.



Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Parameters
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