Plasmid_Backbone

Part:BBa_K3893016

Designed by: Camila Gallegos   Group: iGEM21_Ecuador   (2021-10-21)


BioBricks compatible L4440 plasmid

The L4440 backbone is the standard for producing dsRNA, but it is incompatible with the biological building blocks and does not have transcriptional terminators, thus producing long, nonspecific RNA fragments. Thus it was changed with a PCR overhang that included the RFC10 prefix and suffix in order to convert it to our modular platform afterwards.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found at 2526
    Illegal suffix found at 4
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 2526
    Illegal SpeI site found at 5
    Illegal PstI site found at 19
    Illegal NotI site found at 12
    Illegal NotI site found at 2532
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 2526
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found at 2526
    Illegal suffix found at 5
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found at 2526
    Plasmid lacks a suffix.
    Illegal XbaI site found at 2541
    Illegal SpeI site found at 5
    Illegal PstI site found at 19
    Illegal NgoMIV site found at 2245
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal BsaI.rc site found at 1069


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