Composite

Part:BBa_K389051

Designed by: Jonas Aretz   Group: iGEM10_Bielefeld-Germany   (2010-10-08)


Tightly regulated lacO + luc

This is the tightly regulated lac operon BBa_K389050 with a luciferase gene as readout.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1210
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1690
    Illegal NgoMIV site found at 3034
    Illegal NgoMIV site found at 3055
    Illegal AgeI site found at 2758
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 2940


[edit]
Categories
//chassis/prokaryote/ecoli
//direction/forward
//function/reporter/light
//promoter
//regulation/negative
Parameters
None