Regulatory
VirA/G

Part:BBa_K389017

Designed by: Jonas Aretz   Group: iGEM10_Bielefeld-Germany   (2010-09-28)

VirA/G signaling system without read-out

This BioBrick is a VirA/G signaling system from Agrobacterium tumefaciens without read-out. It contains an unmutated virA gene and a virG gene that works without rpoA from A. tumefaciens and a vir promoter. In the presence of the inducer acetosyringone VirA phosphorylates VirG and this activates the vir promoter.


Parameters

Characterizations of this part were made with BBa_K389015 and BBa_K389016. Look at the description of these parts to get more detailed information about the VirA/G signaling system. In the following table, some important parameters of the VirA/G signaling system are summarized:

Parameter Result
Input Acetosyringone
Output PoPS
Switch point ~ 30 µM acetosyringone
Fully induced > 150 µM acetosyringone
Increased expression when induced > 2.2 fold (compared to uninduced system)
Hill coefficient positive cooperation (> 1)
Not induced by

Capsaicin
Dopamine
Homovanillic acid
3-Methoxytyramine


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal NheI site found at 647
    Illegal NheI site found at 2581
    Illegal NheI site found at 2604
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1632
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 1768


[edit]
Categories
//cds/membrane/receptor
//cds/transcriptionalregulator/activator
//chassis/prokaryote/ecoli
//promoter
//regulation/positive
Parameters
None