Part:BBa_K389001
VirA receptor
The VirA receptor is used by Agrobacterium tumefaciens to detect acetosyringone and other phenolic substances which are secreted by plants after injury. In presence of these substances VirA phosphorylates itself and afterwards VirG, a response regulator which activates vir promoters. These promoters control genes which are used for infecting the injured plant.
This virA gene was isolated from the TI-plasmid of A. tumefaciens C58. An illegal PstI restriction site was removed by site-directed mutagenesis.
Usage and Biology
For a working VirA/G-receptor system, you also need the virG BioBrick BBa_K389002 and a vir promoter BBa_K389003.
You can find an already assembled VirA/G-receptor system here: BBa_K389017.
Specifity
The functionality and specifity of this BioBrick were tested with the devices: BBa_K389015, BBa_K389016.
This receptor recognizes:
- Acetosyringone
This receptor does not recognize
- Capsaicin
- Dopamine
- Homovanillic acid
- 3-Methoxytyramine
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 586
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1571
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 1707
//cds/receptor
//chassis/prokaryote/ecoli
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