Part:BBa_K3890006

CYP6G1 CDS

The CYP6G1 coding sequence express the CYP6G1 enzyme responsible for metabolization of neonicotinoids, as imidacloprid pesticide [2]. It was found in pesticide resistant Drosophila melanogaster, and its codons were here optimized for tomato expression.

Usage and Biology

CYP6G1 is part of the CYP P450 mono-oxygenases superfamily, responsible for the metabolization of a extense range of chemicals, from drugs, pesticides, steroids and fatty acids [1].

Drosophila melanogaster that are resistant to DDT have been reported to overexpress by 10 to 100 times the CYP6G1. This overtranscription has been correlated to their resistance to Imidacloprid and assays with the enzyme shows it is very effective in metabolizing the phytosanitary product. In 48h it metabolized 83% of the imidacloprid pesticide, from the neonicotinoid family (usually associated with the worldwide bees’ disappearance phenomenon), to less harmful metabolites 4-hydroxyimidacloprid and 5-hydroxyimidacloprid [2].

Structure

To understand the interactions between imidacloprid and the catalytic site of the enzyme, we needed to know its structure. But there was no corroborated structure to be found in data banks, thus we had to model it through in silico programs, using CYP3A4 as a template. More abou the CYP's structure can be found at Team USP-Brazil 2021 wiki: https://2021.igem.org/Team:USP-Brazil/Model

Figure 1. A modelled CYP6g1 catalytic site.

References

1. INTERPRO. Cytochrome P450 superfamily. Retrieved in October 20th 2021 from https://www.ebi.ac.uk/interpro/entry/InterPro/IPR036396/

2. JOUßEN, N., Heckel, D. G., Haas, M., Schuphan, I., & Schmidt, B. (2007). Metabolism of imidacloprid and DDT by P450 CYP6G1 expressed in cell cultures of Nicotiana tabacum suggests detoxification of these insecticides inCyp6g1-overexpressing strains ofDrosophila melanogaster, leading to resistance. Pest Management Science, 64(1), 65–73. doi:10.1002/ps.1472

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI site found at 1416
  Illegal SapI site found at 1246