Composite

Part:BBa_K3889111

Designed by: Prameya Garge, Ashwin Sharma   Group: iGEM21_IISER-Tirupati_India   (2021-10-12)


Bacillus Subtilis yqcG toxin casette

This gene cassette has been designed to be a part of the genetic circuit for an inducible kill switch in Bacillus subtilis. The gene coding for the YqcG toxin (BBa_K3889025) is downstream to the xylose inducible promoter BBa_K143014. The cassette is complete with the double terminator (BBa_B0015).

The cassette has been designed to be a part of a genetic circuit of a xylose inducible kill switch. The inducible production of YqcG will tune the death of Bacillus subtilis.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
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