Coding

Part:BBa_K3889092

Designed by: Prameya Garge, Ashwin Sharma   Group: iGEM21_IISER-Tirupati_India   (2021-10-08)


XylR repressor(w/o stop codons)

XylR is a xylose based repressor from Bacillus subtilis . It is a part of XylR regulon where it functions to regulate xylose utilization. It can repress Pxyl (BBa_K143014) in absence of xylose.

This is a modified version of XylR (BBa_K143036) from which stop codon is omitted so that it can be fused with P2A linker (BBa_K3889069) to coexpress fluorescent proteins and report protein production.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None