Coding

Part:BBa_K3889028

Designed by: Prameya Garge, Ashwin Sharma   Group: iGEM21_IISER-Tirupati_India   (2021-10-02)


Modified mfLon protease

mfLon is a Lon protease present in most bacteria from Mesoplasma florum. It can function independently of the host, providing functionality in a variety of bacteria. It can degrade proteins at different rates depending on the protein degradation tag attached to it [1].


This is a modified version of BBa_K3889020 specific to our project's assembly. Mutated multiple RE sites to make it assembly compatible.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


References

1. Cameron, D. E., & Collins, J. J. (2014). Tunable protein degradation in bacteria. Nature biotechnology, 32(12), 1276–1281. https://doi.org/10.1038/nbt.3053

[edit]
Categories
Parameters
None