RNA

Part:BBa_K3886016

Designed by: Yujie Zhao & Fankang Meng   Group: iGEM21_NDNF_China   (2021-10-13)


Targeting-Barcode(BBa_K3886005) crRNA

The crRNA here is an RNA sequence that leads CRISPR-Cas12a protein to target a barcode named the "NDNFCHINAiGEM2021 Barcode (BBa_K3886005)" on the E. coli genome.


Characterization

Once the Hidro system is performing a task or finishing a task, we can apply the crRNA to perform the detection assay to know whether there are any escape events happening. CRISPR-Cas12a could efficiently detect the strain escaping from Hidro. A band will be shown on a test strip when the CRISPR-Cas12a-crRNA complex detects the barcode on the E. coli genome (Figure 1). For more information about Hidro, please visit: NDNF_China Description.

Figure 1: A band is shown on a test strip when CRISPR-Cas12a-crRNA complex detects the barcode on the E. coli genome.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 18
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Parameters
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