Composite

Part:BBa_K386003

Designed by: Matthew Ford   Group: iGEM10_Sheffield   (2010-10-25)

HapR promoter with GFP

This composite contains the HapR promoter with GFP down stream of it contained in plasmid pSB1C3 (Cm resistant). HapR is a downstream product of the CqsS pathway found in the cholera quorum sensing system. HapR acts to inhibit virulence genes in cholera when there is a high cell density.

This part can be used as a reporter of the CqsS cholera quorum sensing system.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 785


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Categories
Parameters
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