DNA

Part:BBa_K3860009

Designed by: Marius Schnutenhaus   Group: iGEM21_RUBochum   (2021-09-30)


Acetyl carborxylases complex from e. coli k12

The Acetyl-coA carboxylase complex was isolated from e. coli K12 and subunits were conneceted with glycin linkers.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 3562
    Illegal PstI site found at 2671
    Illegal PstI site found at 2713
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 3562
    Illegal PstI site found at 2671
    Illegal PstI site found at 2713
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 3562
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 3562
    Illegal PstI site found at 2671
    Illegal PstI site found at 2713
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 3562
    Illegal PstI site found at 2671
    Illegal PstI site found at 2713
    Illegal AgeI site found at 579
    Illegal AgeI site found at 1404
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 517
    Illegal SapI.rc site found at 714


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Categories
Parameters
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