DNA

Part:BBa_K3860001

Designed by: Marius Schnutenhaus   Group: iGEM21_RUBochum   (2021-09-30)


αS2-casein from Bos taurus codon optimized for P. pastoris and secretion signal

The αS2-casein from Bos taurus was optimized for P. pastoris. For secretion the α secretion signal was added.


Usage and Biology

Used for the production and secretion of αS2-casein (bovine) in P.pastoris Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 809
    Illegal BamHI site found at 890
    Illegal XhoI site found at 244
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None