Composite

Part:BBa_K3857003

Designed by: Valeria Martínez De León   Group: iGEM21_RUM-UPRM   (2021-10-20)


Luxpr promoter fitted with xplB and xplA, followed by amilGFP

This device was created for the purpose of RDX biodegradation. This device contains the luxpr promoter, which can be activated in presence of luxR in complex with HSL and initiate transcription. Gene expression will then produce the RDX-metabolizing cytochrome P450, xplA, and accompanying reductase, xplB. amilGFP will serve as the gene reporter for this sequence.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 337
    Illegal BamHI site found at 1861
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None