Plasmid_Backbone

Part:BBa_K3855401

Designed by: Sebastian Bayer   Group: iGEM21_BOKU-Vienna   (2021-09-27)


Backbone 3 A-B for Type 2 s assembly in Lactobacillus plantarum

Plasmidbackbone for type II s assembly that works in E.coli and Lactobacillus.

Includes Pre and Suffix accordingly ( http://parts.igem.org/partsdb/scars.cgi). Allows for an easy host switch between E.Coli and Lactobacillus as it works in both organisms. It contains an eryhtromycin resistance and annotations for two primers. Those are meant for sequencing of inserts. Build from pisp 403.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found at 1
    Illegal suffix found at 5227
    Illegal SpeI site found at 4907
    Illegal PstI site found at 4941
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 1
    Illegal SpeI site found at 4907
    Illegal SpeI site found at 5228
    Illegal PstI site found at 4941
    Illegal PstI site found at 5242
    Illegal NotI site found at 7
    Illegal NotI site found at 5235
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 1
    Illegal BamHI site found at 1548
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found at 1
    Illegal suffix found at 5228
    Illegal SpeI site found at 4907
    Illegal PstI site found at 4941
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found at 1
    Plasmid lacks a suffix.
    Illegal XbaI site found at 16
    Illegal SpeI site found at 4907
    Illegal SpeI site found at 5228
    Illegal PstI site found at 4941
    Illegal PstI site found at 5242
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal BsaI site found at 23
    Illegal BsaI.rc site found at 5221


[edit]
Categories
Parameters
None