PrRNA11- strong lactobacilli promotor
This part contains a strong constitutive promoter from Lactobacillus plantarum. A GusA activity of approximately of 1600 MU.
We confirmed the activity using mCherry as signalling protein. Simultaneously, we compared it to 7 other promotors. Five of those we added to the registry and where published by Rud et al 2006 . Two were published by Team Austin, Texas, 2017. We showed that the strength exceeds all other promotors by far. Even though BBa_K2253000 is described as a “strong” promotor, this novel part BBa K3855000 exceeded it 4-fold.
Experimental set up
5ml cultures of the eight different transfected Lactobacillus plantarum NC8, as well as an untransfected control, were incubated at 30°C overnight under anaerobic conditions and constant shaking. On the following day, conditions were changed to aerobic for 3-4 hours. Afterwards, the cell suspensions were diluted to different densities (OD 600 of 2, 1 and 0.1). The measurement was carried out using a Tecan reader. Samples where excited at 587 nm and emission measured at 640 nm. Sterile media was used as a reference. Experiments were carried out in triplets.
We found that the promotor expression is of very high strength. All other promotors were significantly weaker.
Rud et al. 2006: A synthetic promoter library for constitutive gene expression in Lactobacillus plantarum MICROBIOLOGY Volume 152, Issue 4.
Sequence and Features
- 10COMPATIBLE WITH RFC
- 12COMPATIBLE WITH RFC
- 21COMPATIBLE WITH RFC
- 23COMPATIBLE WITH RFC
- 25COMPATIBLE WITH RFC
- 1000COMPATIBLE WITH RFC