Tag

Part:BBa_K3853019

Designed by: Peng Luo   Group: iGEM21_CPU_CHINA   (2021-09-29)


his-tag Codon adapted for Pichia pastoris

This part is used to encode a short peptide containing six histidines for protein purification. Using the Thermo GeneArt online tool geneoptimize, we optimize the codon of the peptide gene sequence to improve its expression in Pichia pastoris.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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