DNA

Part:BBa_K3853007

Designed by: Gongyu Liu   Group: iGEM21_CPU_CHINA   (2021-09-20)


gRNA scaffold

This is a sequence used to generate sgRNA. Through this part, we can use primers containing different target sequences to generate different sgRNA. They can bind to different sites of the dsDNA produced by BBa_K3853006. We used this part to generate three kinds of sgRNA.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None