Coding

Part:BBa_K3853004

Designed by: Peng Luo   Group: iGEM21_CPU_CHINA   (2021-09-19)


SpyTag Codon adapted for Pichia pastoris

This part codes for a protein that recognizes and forms a covalent isopeptide bound to a oligopeptide, called SpyTag. That oligopeptide can be found under BBa_K3853005. This part codes the same protein as BBa_K1159200. This is a codon-optimized version to improve its expression in Pichia pastoris GS115.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None