Coding

Part:BBa_K385004

Designed by: Krystal Annand, Joseph Hoare, Stephen Lam, Justyna Kucia   Group: iGEM10_Aberdeen_Scotland   (2010-09-27)


Phage lambda N-peptide, tandem repeat

Two copies of the N-peptide from phage lambda, arranged as a tandem repeat. The N-peptide protein coding sequence functions in a phage transcriptional termination control mechanism, by binding to an RNA stem loop (B-box) in a sequence specific manner. This peptide can be used as part of a translational control strategy for eukaryote gene expression. The B-box sequence should be placed in the 5' leader of a gene whose expression is to be controlled, and the N-peptide is expressed in trans to regulate ribosomal scanning. Tandem repeats of the N-peptide were cloned in this BioBrick so as to optimise binding opportunities to the target mRNA stem loop.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 163
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 29
    Illegal SapI.rc site found at 116


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Categories
Parameters
None