Part:BBa_K3848003

SMAC/DIABLO (Second Mitochondria-derived Activator of Caspase/Direct Inhibitor of Apoptosis-Binding

SMAC/DIABLO is a pro apoptogenic mitochondrial protein released to the cytosol in response to various apoptotic stimuli. Released SMAC then interacts and bypasses inhibitors of apoptosis proteins (IAPs), thus allowing the activation of caspases and apoptosis. Furthermore, SMAC peptides also sensitize tumor cells for apoptosis induced by death-receptor ligation or cytotoxic drugs.

In our project, we specifically used SMAC-N7 (the seven N-terminal amino acids AVPIAQK of the mitochondrial protein SMAC) to enhance the antitumor activity of TRAIL(Tumor necrosis factor-Related Apoptosis-Inducing Ligand).

References

1.Fulda S, Wick W, Weller M, Debatin KM. Smac agonists sensitize for Apo2L/TRAIL- or anticancer drug-induced apoptosis and induce regression of malignant glioma in vivo. Nat Med. 2002 Aug;8(8):808-15. doi: 10.1038/nm735. Epub 2002 Jul 15. PMID: 12118245.

2.Heckl S, Sturzu A, Regenbogen M, Beck A, Feil G, Gharabaghi A, Echner H. A novel polyarginine containing Smac peptide conjugate that mediates cell death in tumor and healthy cells. Med Chem. 2008 Jul;4(4):348-54. doi: 10.2174/157340608784872217. PMID: 18673146.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]