Regulatory

Part:BBa_K3842018

Designed by: Xiaoyi Feng   Group: iGEM21_GA_State_SW_Jiaotong   (2021-10-18)


Lac promoter

This lac promoter includes a CAP binding site and the lac operator sequence lacO. The lac promoter is not as strong as the tac or the trc promoter, but in high copy-number vectors it allows expression of foreign proteins at respectable levels. In this program, this part is used in our program to regulate Lac I protein which is a repressor for lac operator.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None