Plasmid
pNW33N+la

Part:BBa_K3842010

Designed by: Xiaoyi Feng   Group: iGEM21_GA_State_SW_Jiaotong   (2021-10-17)


pNW33N+lac+ Gas6

This plasmid contains the backbone of pNW33N plasmide, T7 promoter, lac operator, Gas6 coding sequence, T7 terminator.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 4319
    Illegal XbaI site found at 4186
    Illegal PstI site found at 4174
    Illegal PstI site found at 5264
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 4319
    Illegal NheI site found at 6395
    Illegal PstI site found at 4174
    Illegal PstI site found at 5264
    Illegal NotI site found at 6380
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 4319
    Illegal BglII site found at 4830
    Illegal BamHI site found at 4192
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 4319
    Illegal XbaI site found at 4186
    Illegal PstI site found at 4174
    Illegal PstI site found at 5264
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 4319
    Illegal XbaI site found at 4186
    Illegal PstI site found at 4174
    Illegal PstI site found at 5264
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 548
    Illegal BsaI.rc site found at 2239
    Illegal SapI.rc site found at 1630


[edit]
Categories
//plasmid/expression
Parameters
None