Part:BBa_K3841006

Venus Yellow Fluorescent Protein for yeast

Venus yellow fluorescent protein from Aequorea victoria [1], codon-optimized for expression in Komagataella phaffii (previously referred to as Pichia Pastoris). The protein is suitable to use as a fluorescent reporter. If used as fusion tag it is recommended to add a linker between Venus and the other protein domain not to impair the activity [2].

Venus has an excitation wavelength of 515 nm and emission wavelength of 528 nm [3]. Venus has the advantage over many other YFPs of being less sensitive to environmental factors such as pH and chloride ions, and has a quicker rate of maturation [1].

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

References

[1] Nagai, Takeharu, et al. “A Variant of Yellow Fluorescent Protein with Fast and Efficient Maturation for Cell-Biological Applications.” Nature Biotechnology, vol. 20, no. 1, NATURE PUBLISHING GROUP, 2002, pp. 87–90, doi:10.1038/nbt0102-87.

[2] Chen, Xiaoying, et al. “Fusion Protein Linkers: Property, Design and Functionality.” Advanced Drug Delivery Reviews, vol. 65, no. 10, ELSEVIER SCIENCE BV, 2013, pp. 1357–69, doi:10.1016/j.addr.2012.09.039.

[3] Shaner, Nathan C., et al. “A Guide to Choosing Fluorescent Proteins.” Nature Methods, vol. 2, no. 12, NATURE PUBLISHING GROUP, 2005, pp. 905–09, doi:10.1038/nmeth819.