Plasmid

Part:BBa_K3839041

Designed by: Georgios Psarias   Group: iGEM21_Patras   (2021-10-21)


pENTR/D-TOPO vector + CYB5A_202

To directionally clone a blunt-end PCR product into a vector, the pENTR Directional TOPO Cloning approach uses a highly efficient 5-minute cloning strategy ("TOPO Cloning"). The transcript CYB5A_202 cDNA, that contains a TOPO binding site at the 5' end of the sequence, was cloned into the vector.



Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 1044
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 239
    Illegal NheI site found at 505
    Illegal PstI site found at 1044
    Illegal NotI site found at 671
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 1044
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 1044
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 116
    Illegal SapI.rc site found at 812
[edit]
Categories
Parameters
None