Plasmid

Part:BBa_K3839040

Designed by: Georgios Psarias   Group: iGEM21_Patras   (2021-10-21)



pENTR/D-TOPO vector + POR-218


To directionally clone a blunt-end PCR product into a vector, the pENTR Directional TOPO Cloning approach uses a highly efficient 5-minute cloning strategy ("TOPO Cloning"). The transcript POR-218 cDNA, that contains a TOPO binding site at the 5' end of the sequence, was cloned into the vector.



Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 966
    Illegal PstI site found at 1163
    Illegal PstI site found at 1548
    Illegal PstI site found at 1988
    Illegal PstI site found at 2048
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 239
    Illegal NheI site found at 505
    Illegal PstI site found at 966
    Illegal PstI site found at 1163
    Illegal PstI site found at 1548
    Illegal PstI site found at 1988
    Illegal PstI site found at 2048
    Illegal NotI site found at 671
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 966
    Illegal PstI site found at 1163
    Illegal PstI site found at 1548
    Illegal PstI site found at 1988
    Illegal PstI site found at 2048
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 966
    Illegal PstI site found at 1163
    Illegal PstI site found at 1548
    Illegal PstI site found at 1988
    Illegal PstI site found at 2048
    Illegal NgoMIV site found at 1369
    Illegal NgoMIV site found at 1488
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 803
    Illegal BsaI.rc site found at 1125
    Illegal BsaI.rc site found at 2132
    Illegal SapI.rc site found at 116
    Illegal SapI.rc site found at 1495
[edit]
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