Plasmid

Part:BBa_K3839035

Designed by: Georgios Psarias   Group: iGEM21_Patras   (2021-10-21)


pENTR/D-TOPO

To directionally clone a blunt-end PCR product into a vector, the pENTR Directional TOPO Cloning approach uses a highly efficient 5-minute cloning strategy ("TOPO Cloning").

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 239
    Illegal NheI site found at 505
    Illegal NotI site found at 671
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 116


[edit]
Categories
Parameters
None