Primer

Part:BBa_K3839008

Designed by: Georgios Psarias   Group: iGEM21_Patras   (2021-10-20)


Forward mutagenic primer CYP2C19 E274G

The Forward mutagenic primer that hybridized with the insert cDNA CYP2C19 on the pENTR/D-TOPO vector with one miss-match. The substitution was created by incorporating the desired nucleotide change in the center of the forward primer, including at least ten (10) complementary nucleotides on the 3´side of the mutation(s). The reverse primer was designed so that the 5´ ends of the two primers anneal back-to- back. This certain primer utilized the change of A to G on the cDNA, resulting in the change E274G on the amino-acid sequence of CYP2C19.



Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]
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Categories
Parameters
None