Measurement

Part:BBa_K3832009

Designed by: Hongyu Chen   Group: iGEM21_XJTU-China   (2021-10-21)


trp-induce (detection circuit of tryptophan)

This part is used to detect the concentration of tryptophan in the environment. A reporter gene (sfGFP) are contained in the part, following a inducible promoter trpP and leader peptide CDS trpL. With the present of trpR, the expression of sfGFP can be inhibited by high concentration of tryptophan. trpL, as the repressor, can further inhibit the expression of downstreaming genes. As a consequence, the intensity of sfGFP fluorescence decreases with the increase of tryptophan concentration.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 1091
    Illegal SpeI site found at 1099
    Illegal SpeI site found at 2077
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 1091
    Illegal SpeI site found at 1099
    Illegal SpeI site found at 2077
    Illegal NotI site found at 2084
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 1091
    Illegal SpeI site found at 1099
    Illegal SpeI site found at 2077
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 1091
    Illegal SpeI site found at 1099
    Illegal SpeI site found at 2077
    Illegal AgeI site found at 915
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 490
    Illegal SapI.rc site found at 1317


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Categories
Parameters
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