Part:BBa_K3832004

medium RBS

This part is a medium RBS in E.coli, which has been characterized by Timothy S. Gardner & James J. Collins[1]. It can be used to construct a bistable toggle switch circuit. The efficiency data can be seen in the Reference (RBS-D in the article)

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal AgeI site found at 6
  
• 1000
  COMPATIBLE WITH RFC[1000]

Reference

[1]Timothy S. Gardner, Charles R. Cantor, James J. Collins, Construction of a genetic toggle switch in Escherichia coli Supplementary Information, Nature volume 403, pages 339–342 (2000)