Composite

Part:BBa_K3831033

Designed by: Barbora Hrnčířová   Group: iGEM21_Brno_Czech_Republic   (2021-10-07)


Construct D for testing the function of modified Pgrac promoter for expression of cI repressor

We designed this composite part with the aim to test the function of the newly created promoter Pgrac OcI in Bacillus subtilis and its response to the presence of the cI repressor originating from phage lambda.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 2699
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 2898
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 228
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//cds
//chassis/prokaryote/bsubtilis
//function/reporter
Parameters
chassisBacillus subtilis