Composite

Part:BBa_K3831032

Designed by: Michaela Dušková   Group: iGEM21_Brno_Czech_Republic   (2021-10-07)


Construct C for testing of Ppho promotor - detecting of phosphate and production of cI repressor

We designed this composite part to test the function of Ppho promoter in Bacillus subtilis. Ppho is a native promoter of B. subtilis. It is activated by the Pho signaling pathway which is in turn activated by low phosphate concentrations. This promoter was described in a study by Vershinina & Znamenskaia in 2002 [1]. We used the predicted sequence from this study from the 5' end of the promoter to the start codon of the first protein in the native operon sequence. Reporter protein mScarlet-I has been added downstream of this promoter to monitor its function. The cI repressor, which is tested by construct D (BBa_K3831033), has also been placed under the control of Pho promotor.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 2301
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 636
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//cds
//chassis/prokaryote/bsubtilis
//function/structures
Parameters
chassisBacillus subtilis