Regulatory

Part:BBa_K3831025

Designed by: Barbora Hrnčířová   Group: iGEM21_Brno_Czech_Republic   (2021-10-07)


Pgrac OcI promoter

The Pgrac promoter was created by combining the groE promoter, gsiBSD sequence and lacO operator to allow for IPTG induction. We took out the lacO sequence and instead of it we inserted the cI operator into the promoter sequence. This design should allow the strong promoter to constitutively express the gene downstream of it until the expression is stopped by the cI repressor originating from Phage lambda.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//promoter
Parameters
chassisBacillus subtilis