Part:BBa_K3830017

pGET-118 inserted with celK, T7 terminator and pr promoter

we use this plasmid to hydrolyze the reducing or non-reducing end to xylan

disaccharide and we inserted pr promoter to enhance the express of this plasmid

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal EcoRI site found at 1841
  Illegal EcoRI site found at 1909
  Illegal XbaI site found at 116
  Illegal PstI site found at 699
  Illegal PstI site found at 1176
  Illegal PstI site found at 2061
  Illegal PstI site found at 2100
  Illegal PstI site found at 2466
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal EcoRI site found at 1841
  Illegal EcoRI site found at 1909
  Illegal NheI site found at 2850
  Illegal PstI site found at 699
  Illegal PstI site found at 1176
  Illegal PstI site found at 2061
  Illegal PstI site found at 2100
  Illegal PstI site found at 2466
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal EcoRI site found at 1841
  Illegal EcoRI site found at 1909
  Illegal BglII site found at 1
  Illegal BamHI site found at 1522
  Illegal XhoI site found at 467
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal EcoRI site found at 1841
  Illegal EcoRI site found at 1909
  Illegal XbaI site found at 116
  Illegal PstI site found at 699
  Illegal PstI site found at 1176
  Illegal PstI site found at 2061
  Illegal PstI site found at 2100
  Illegal PstI site found at 2466
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal EcoRI site found at 1841
  Illegal EcoRI site found at 1909
  Illegal XbaI site found at 116
  Illegal PstI site found at 699
  Illegal PstI site found at 1176
  Illegal PstI site found at 2061
  Illegal PstI site found at 2100
  Illegal PstI site found at 2466
  Illegal AgeI site found at 1286
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal SapI.rc site found at 2126