Coding
Part:BBa_K3830013
Designed by: Fang-Ti Group: iGEM21_NCHU_Taichung (2021-10-18)
celK is exoglycosidase
After fiber has been hydrolyzed, exo-1,4-β-D-glucanase starts to hydrolyze the reducing or non-reducing end to xylan disaccharide Finally, β-glucosidase hydrolyze xylan disaccharide to glucose
Figure 1. The electropherogram of celA, celK, xynC and cipA
Figure 2. 3D structure of celK.
Figure 3. 2D structure of celK.
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 1687
Illegal EcoRI site found at 1755
Illegal PstI site found at 545
Illegal PstI site found at 1022
Illegal PstI site found at 1907
Illegal PstI site found at 1946
Illegal PstI site found at 2312 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 1687
Illegal EcoRI site found at 1755
Illegal PstI site found at 545
Illegal PstI site found at 1022
Illegal PstI site found at 1907
Illegal PstI site found at 1946
Illegal PstI site found at 2312 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 1687
Illegal EcoRI site found at 1755
Illegal BamHI site found at 1368
Illegal XhoI site found at 313 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 1687
Illegal EcoRI site found at 1755
Illegal PstI site found at 545
Illegal PstI site found at 1022
Illegal PstI site found at 1907
Illegal PstI site found at 1946
Illegal PstI site found at 2312 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 1687
Illegal EcoRI site found at 1755
Illegal PstI site found at 545
Illegal PstI site found at 1022
Illegal PstI site found at 1907
Illegal PstI site found at 1946
Illegal PstI site found at 2312
Illegal AgeI site found at 1132 - 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 1972
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