Composite

Part:BBa_K3827004

Designed by: Livia Vieira   Group: iGEM21_British_Columbia   (2021-10-20)


pLactate - luxCDE - lldR

pLactate promoter upstream of lux operon genes luxC, luxD, and luxE. psicA is a promoter that is part of the lactate operon in Salmonella; regulated by Lldr.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 3881
    Illegal NheI site found at 3904
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2165
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1571
    Illegal SapI.rc site found at 2697


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Categories
Parameters
None