Composite

Part:BBa_K3826007

Designed by: Lucas Müller   Group: iGEM21_Duesseldorf   (2021-10-21)


promoter pRha mVenus activator rhaS

The activator rhaS increases the transcription of the L-rhamnose-inducible promoter (pRha). This increases the expression of the fluorescent protein mVenus. Originally, the activator rhaS could only be found in the genome of E.coli. When integrated into the plasmid, however, this system can be applied to many other organisms.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 132
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1291
    Illegal BamHI site found at 758
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 156
    Illegal AgeI site found at 279
    Illegal AgeI site found at 1023
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None