Result

According to the advice given by Dr. Xiaoqing, "Multiple genetic testing can improve the accuracy and sensitivity of the product, but too many genes will bring about cost increase, complexity, and other negative effects". We decided to add another gene SDC2，together with TFPI2， as a detector to determine whether it is colorectal cancer.

Same to TFPI2, we conducted a lot of literature research, selected a methylation-enriched region of SDC2 and designed methylation-specific primers. T7 promoter was added to its forward primer and trigger primer was added to its reverse primer. The primer we designed are below:

SDC2-M: methylation specific primer

F: 5′- TAATACGACTCACTATAGGGGTGTAGAAATTAATAAGTGAGAGGG-3′

R: 5′- TATGTAATTGATTTGGCTTCTGTTAGTTTCATATTTCTACTTACAACCAAAACAAAAC-3′

PCR product：264bp (The red is T7 and the blue is Trigger)

Due to time reasons, we did not conduct experimental verification, but the exact same experiment as TFPI2 determines the success of the experiment.

Part:BBa_K3822004

Result