Composite

Part:BBa_K3817017

Designed by: Sun Ji   Group: iGEM21_UCAS-China   (2021-10-01)


SAM2-CBS inserted by Bleomycin pathway

This pathway is used for the complete and efficient output of SAM, and through yeast production, we obtain the corresponding products for use as cofactors in subsequent reactions.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1361
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1361
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1361
    Illegal BglII site found at 1375
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1361
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1361
    Illegal NgoMIV site found at 2047
    Illegal NgoMIV site found at 2108
    Illegal AgeI site found at 751
    Illegal AgeI site found at 1408
    Illegal AgeI site found at 2655
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 495
    Illegal BsaI.rc site found at 1032


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Categories
Parameters
None