Composite

Part:BBa_K3817014

Designed by: Sun Ji   Group: iGEM21_UCAS-China   (2021-10-01)


Cdh pathway

This part allows for a complete gene pathway. Transferring this gene pathway into the plasmid and transferring the plasmid into E. coli can complete the efficient expression of the enzyme in E. coli.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 350
    Illegal XbaI site found at 47
    Illegal PstI site found at 2982
    Illegal PstI site found at 3535
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 350
    Illegal NheI site found at 133
    Illegal NheI site found at 2074
    Illegal PstI site found at 2982
    Illegal PstI site found at 3535
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 350
    Illegal BglII site found at 239
    Illegal XhoI site found at 1039
    Illegal XhoI site found at 2308
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 350
    Illegal XbaI site found at 47
    Illegal PstI site found at 2982
    Illegal PstI site found at 3535
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 350
    Illegal XbaI site found at 47
    Illegal PstI site found at 2982
    Illegal PstI site found at 3535
    Illegal AgeI site found at 777
    Illegal AgeI site found at 1269
    Illegal AgeI site found at 1437
    Illegal AgeI site found at 1672
    Illegal AgeI site found at 1912
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None