Composite

Part:BBa_K3817012

Designed by: Sun Ji   Group: iGEM21_UCAS-China   (2021-10-01)


CkTcS new_pathway

This part allows for a complete gene pathway. Transferring this gene pathway into the plasmid and transferring the plasmid into E. coli can complete the efficient expression of the enzyme in E. coli.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 350
    Illegal XbaI site found at 47
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 350
    Illegal NheI site found at 133
    Illegal NheI site found at 438
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 350
    Illegal BglII site found at 239
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 350
    Illegal XbaI site found at 47
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 350
    Illegal XbaI site found at 47
    Illegal NgoMIV site found at 854
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None