Composite

Part:BBa_K3817011

Designed by: Sun Ji   Group: iGEM21_UCAS-China   (2021-10-01)


CkTcS pathway

This part allows for a complete gene pathway. Transferring this gene pathway into the plasmid and transferring the plasmid into E. coli can complete the efficient expression of the enzyme in E. coli.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 358
    Illegal XbaI site found at 47
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 358
    Illegal NheI site found at 141
    Illegal NheI site found at 452
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 358
    Illegal BglII site found at 247
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 358
    Illegal XbaI site found at 47
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 358
    Illegal XbaI site found at 47
    Illegal NgoMIV site found at 871
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None