Coding

Part:BBa_K3817005

Designed by: Sun Ji   Group: iGEM21_UCAS-China   (2021-10-01)


Ptrc temp-sensitive promoter

Ptrc is a temperature control promoter module. After mutation induction, the promoter can achieve complete repression at below 30 ℃, and if added upstream of the antitoxin gene can achieve low temperature control.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 78
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 78
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 78
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 78
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None